Genetic analysis and penicillin-binding protein alterations in Neisseria gonorrhoeae with chromosomally mediated resistance.
نویسنده
چکیده
Eight recent isolates of Neisseria gonorrhoeae with high-level non-beta-lactamase-mediated penicillin resistance were investigated. The penicillin-binding proteins of these strains were found to have reduced affinity for radiolabeled penicillin. Testing for known resistance genes showed that these were present in the resistant isolates. Genetic transformation was used to construct strains with increasing levels of antibiotic resistance. Modification of the transformation protocol made it possible to isolate transformants at the highest (penicillin-resistant DNA donor) level of resistance. These transformants unexpectedly yielded two distinct penicillin-binding protein patterns when tested.
منابع مشابه
A novel real-time duplex PCR assay for detecting penA and ponA genotypes in Neisseria gonorrhoeae: Comparison with phenotypes determined by the E-test.
BACKGROUND For many years, the pathogenic bacterium Neisseria gonorrhoeae, the etiologic agent of gonorrhea, was generally susceptible to penicillin, until the emergence of resistant strains. Well-characterized genetic variations in the penicillin resistance-determining region correlate with decreased susceptibility to penicillin. At least 5 genes (penA, penB, mtrR, ponA, and penC) are involved...
متن کاملResistance to β-Lactams in Neisseria ssp Due to Chromosomally Encoded Penicillin-Binding Proteins
Neisseria meningitidis and Neisseria gonorrhoeae are human pathogens that cause a variety of life-threatening systemic and local infections, such as meningitis or gonorrhoea. The treatment of such infection is becoming more difficult due to antibiotic resistance. The focus of this review is on the mechanism of reduced susceptibility to penicillin and other β-lactams due to the modification of c...
متن کاملIdentification and analysis of amino acid mutations in porin IB that mediate intermediate-level resistance to penicillin and tetracycline in Neisseria gonorrhoeae.
PenB is the third resistance determinant in the stepwise acquisition of multiple resistance genes in chromosomally mediated resistant Neisseria gonorrhoeae (CMRNG). Alterations in por(IB), one of two alleles at the por locus that encodes the outer membrane protein porin IB (PIB), were recently reported to be responsible for the increased resistance to penicillin and tetracycline conferred by pe...
متن کاملAntimicrobial resistance of Neisseria gonorrhoeae and high prevalence of ciprofloxacin-resistant isolates in Japan, 1993 to 1998.
To assess the antimicrobial resistance of Neisseria gonorrhoeae isolated from 1993 through 1998 in Japan, susceptibility testing was conducted on 502 isolates. Selected isolates were characterized by auxotype and analysis for mutations within the quinolone resistance-determining region (QRDR) in the gyrA and parC genes, which confer fluoroquinolone resistance on the organism. Plasmid-mediated p...
متن کاملEpidemiology of penicillin resistant Neisseria
Objective-To study the epidemiology of Neisseria gonorrhoeae that exhibit both chromosomal and plasmid-mediated resistance to penicillin. Materials and methods-A total of 1589 strains of N gonorrhoeae isolated from patients attending St Mary's Hospital, London were tested for both their susceptibility to penicillin and for their auxotype and serotype. Results-Of the 940 non-penicillinase produc...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 30 5 شماره
صفحات -
تاریخ انتشار 1986